Sains Malaysiana 40(5)(2011): 475–479

Survivin Expression in Uterine Cervical Exfoliative Cells as Diagnostic Test of Cervical Malignancy Process

(Pernyataan Survivin dalam Sel-sel Eksfoliatif Pangkal Rahim sebagai Ujian Diagnostik daripada Proses-proses Malignan Pangkal Rahim)

Zulham*

Department of Histology, Faculty of Medicine, University of Sumatera Utara

Jl. Dr. Mansur No. 5 Medan 20155 Sumatera Utara, Indonesia

Ahmad Aulia Jusuf

Department of Histology, Faculty of Medicine, University of Indonesia

Jl. Salemba Raya No. 6 Jakarta 10430, Indonesia

Junita Indarti

Dr. Cipto Mangunkusumo Hospital, Department of Obstetrics and Gynecology

Faculty of Medicine, University of Indonesia

Jl. P. Diponegoro No. 71 Jakarta 1043, Indonesia

Lisnawati

Dr. Cipto Mangunkusumo Hospital, Department of Anatomic Pathology

Faculty of Medicine, University of Indonesia

Jl. P. Diponegoro No. 71 Jakarta 10430, Indonesia

Received: 13 August 2009 / Accepted: 11 October 2010

ABSTRACT

Papanicolaou test is a diagnostic test for uterine cervical cancer screening and routinely examined. It has limitations. A better technique is needed to identify true cervical malignancy process. Molecular cancer marker detection may have high the sensitivity and specificity in detecting cancer. Survivin, a marker candidate, is upregulated in many malignancy processes. Ninety women have joined in this cross sectional study by consecutive sampling. Survivin expression was examined by indirect immunoperoxidase method. It was predominantly found in metaplastic cells. Correlation between survivin expression and Papanicolaou test results was calculated by Fischer’s-exact test. Using Papanicolaou test result as an indicator for the presence of uterine cervical abnormalities, the performance indicators were calculated. Fischer’s-exact test showed that survivin expression was not significantly useful as cervical cancer molecular marker. Survivin expression of the uterine cervical exfoliative cells cannot be used as a diagnostic test for uterine cervical malignancy process.

Keywords: Diagnostic test; indirect immunoperoxidase; Papanicolaou test; survivin; uterine cervical cancer

ABSTRAK

Ujian Papanicolau adalah sebuah ujian diagnostik bagi pemeriksaan barah pangkal rahim (kanser serviks) dan dilakukan secara rutin. Ujian ini mempunyai beberapa had. Suatu teknik yang lebih baik diperlukan bagi mengenal pasti proses malignansi serviks yang sebenarnya. Survivin, satu calon penanda kanser, meningkat kadarnya dalam banyak proses malignansi. Sembilan puluh wanita telah menyertai kajian keratan lintang ini melalui pensampelan berturutan. Pernyataan survivin diperiksa dengan menggunakan kaedah imunoperoksidas tidak langsung. Korelasi antara ekspresi survivin dengan keputusan ujian Pap dikira dengan menggunakan ujian Fischer’s-exact. Seterusnya, dengan menggunakan keputusan yang diperoleh daripada ujian Papanicolau sebagai penunjuk adanya keganjilan serviks, penunjuk prestasi daripada immunositokimia daripada survivin boleh dikira. Ujian Fischer’s-exact menunjukkan bahawa ekspresi survivin tidak berguna secara penuh makna sebagai penanda kanser serviks secara molekular. Pernyataan survivin daripada sel-sel eksfoliatif serviks tidak boleh digunakan sebagai ujian diagnostik bagi proses malignan serviks.

Kata kunci: Imunoperoksidas tidak langsung; kanser pangkal rahim; survivin; ujian diagnostik; ujian Papanicolaou

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*Corresponding author; e-mail: dr_zulham@yahoo.com