Sains Malaysiana 43(5)(2014): 683–687

Use of a Single Intramuscular Injection of a Synthetic Hormone Analogue, Ovupin for

Commercial Carp Seed Production in Bangladesh

(Penggunaan Suntikan Tunggal Intraotot Hormon Sintetik Analog, Ovupin untuk Pengeluaran Komersial Benih Kap di Bangladesh)

MD. RASHEDUL KABIR MONDOL*², SHARMIN JAMAN PROVHAT²

& MD. ARIF HOSSAIN³

¹Molecular Ecology Laboratory, Australian Rivers Institute, Nathan Campus, Griffith University

170 Kessels Road, Nathan, QLD-4111, Australia

²Department of Fisheries, University of Rajshahi, Rajshahi-6205, Bangladesh

³Upazila Fisheries Officer, Nimgachi Matsha Hatchery & Training Center, Raigonj, Sirajgong-6720, Bangladesh

Received: 24 December 2012/Accepted: 26 August 2013

ABSTRACT

A study was conducted to investigate the possibility of employing a single intramuscular injection of a synthetic hormone analogue, ovupin on the induced breeding of two major carps, viz. rohu (Labeo rohita), mrigala (Cirrhinus mrigala) and an endangered minor carp, bata (Labeo bata). Three breeding trials of each species were performed. In case of major carp, the females were injected with single dose of ovupin solution at a rate of 0.5 mL kg-1 body weight, while the minor carp received ovupin solution at a dose of 0.3 mL kg-1 body weight, whereas males received extracted PG hormone at a dose of 2 and 1.5 mg kg-1 body weight for major carps and minor carp, respectively. All the three species were successfully bred using ovupin through a single injection. In case of major carps, the latent period was 9-10 h while 12-14 h for minor carp. The breeding response of females was 100% in major carps, whereas it was approximately 90% in minor carp. Fertilization rate varied between 87.07 and 89.94% for the major carps and between 87.6 and 89.9% for minor carps. Major carps showed higher hatching rates (77.21 to 80.19%) than minor carp (64.9 to 66.56%). The present study indicated that ovupin could be effective as alternative of PG in carp’s breeding in Bangladesh.

Keywords: Induced breeding; Linpe method; ovupin; pituitary extract

ABSTRAK

Suatu penyelidikan telah dijalankan untuk mengkaji kemungkinan menggunakan suntikan intraotot tunggal hormon sintetik analog, ovupin pada pembiakbakaan aruhan dua jenis kap utama, viz. rohu (Labeo rohita) dan mrigala (Cirrhinus mrigala) serta kap kecil terancam, bata (bata Labeo). Tiga ujian pembiakan untuk setiap spesies telah dijalankan. Dalam kes kap utama, ikan betina telah disuntik dengan dos tunggal larutan ovupin pada kadar 0.5 mL kg-1 berat badan, manakala kap kecil menerima larutan ovupin pada dos 0.3 mL kg berat badan-1. Ikan jantan pula menerima ekstrak hormon PG pada dos 2 dan 1.5 kg mg-1 berat badan masing-masing untuk kap utama dan kap kecil. Kesemua tiga spesies telah berjaya dibiakkan menggunakan suntikan tunggal ovupin. Dalam kes kap utama, tempoh pendam adalah 9-10 jam manakala 12-14 jam untuk kap kecil. Tindak balas pembiakan ikan betina adalah 100% untuk kap utama dan kira-kira 90% untuk kap kecil. Kadar persenyawaan berbeza antara 87.07 ke 89.94% bagi kap utama dan antara 87.6 dan 89.9% bagi kap kecil. Kap utama menunjukkan kadar penetasan yang lebih tinggi (77.21-80.19%) daripada kap kecil (64.9 kepada 66.56%). Kajian ini menunjukkan bahawa ovupin mampu menjadi alternatif kepada PG dalam pembiakan kap di Bangladesh.

Kata kunci: Ekstrak pituitari; kaedah Linpe; pembiakbakaan aruhan; ovupin

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*Corresponding author; email: rashedul.mondol@griffithuni.edu.au