Sains Malaysiana 43(5)(2014):
715–722
In
vitro Regeneration and Acclimatization Protocols of Selected Ornamental
Plants
(Agapanthus praecox, Justicia betonica and Celosia
cristata)
(Protokol Regenerasi Secara In
vitro dan Aklimatasi Tumbuhan Hiasan Terpilih
(Agapanthus praecox, Justicia
betonica dan Celosia cristata))
JAMILAH SYAFAWATI
YAACOB*1 2, AZANI
SALEH1
3, HASHIMAH
ELIAS1, SAKINAH
ABDULLAH1, NORAINI
MAHMAD1& NORMADIHA
MOHAMED1
1Institute of Biological Sciences, Faculty
of Science, University of Malaya
50603 Kuala Lumpur, Malaysia
2Centre
for Research in Biotechnology for Agriculture (CEBAR), Institute
of Biological Sciences, Faculty of Science, University of Malaya,
50603 Kuala Lumpur, Malaysia
3School of Applied Science, MARA University
of Technology, 40450 Shah Alam, Selangor, Malaysia
Received: 20 May 2013/Accepted: 4
September 2013
ABSTRACT
This paper discussed on the
effectiveness of BAP and NAA growth hormones on
establishment of plant regeneration for selected ornamentals; Agapanthus
praecox, Justicia betonica and Celosia cristata. Various explants
(leaf, stem, shoot tip and bulb) derived from one-month-old aseptic seedlings
of A. praecox and C. cristata, as well as explants from intact
plants of J. betonica were utilized to achieve complete plant
regeneration of these species. MS medium
supplemented with various hormones, with an emphasis on BAP and NAA were
tested to obtain direct and indirect regeneration. Both A. praecox (bulbs)
and C. cristata (shoots) formed complete plantlets on MS added with 0.5 – 2.0 mg/l BAP and NAA,
while direct regeneration was achieved for J. betonica on MS media containing BAP. Several methods were
attempted to acclimatize the regenerants, with A. praecox gave the
highest percentage of survival rates (96.67%), followed by J. betonica (80.00%)
and C. cristata (75.00%).
Keywords: Acclimatization; Agapanthus
praecox; Celosia cristata; Justicia betonica; plant growth regulators
ABSTRAK
Kertas ini menerangkan keberkesanan
hormon pertumbuhan BAP dan NAA terhadap regenerasi tumbuhan
hiasan terpilih; Agapanthus praecox, Justicia betonica dan Celosia cristata.
Pelbagai eksplan (daun, batang, hujung pucuk dan ubi) yang berasal daripada
anak benih aseptik A. praecox dan C. cristata yang berusia satu
bulan, serta eksplan daripada tumbuhan utuh J. betonica telah digunakan
untuk menperoleh regenerasi lengkap spesies ini. Media MS yang dibekalkan dengan pelbagai hormon, dengan penekanan kepada BAP dan NAA telah diuji untuk mendapatkan regenerasi secara langsung atau
tidak langsung. Kedua-dua A. praecox (ubi) dan C. cristata (pucuk)
membentuk anak pokok lengkap di dalam media MS yang ditambah dengan 0.5 - 2.0 mg/L BAP dan NAA,
manakala regenerasi secara langsung telah dicapai oleh J. betonica di
dalam media MS yang mengandungi BAP. Beberapa kaedah telah dicuba
dalam pengikliman anak pokok, dengan A. praecox memberikan peratusan
kadar kelangsungan hidup tertinggi (96.67%), diikuti oleh J. betonica (80.00%)
dan C. cristata (75.00%).
Kata
kunci: Agapanthus
praecox; Celosia cristata; hormon pertumbuhan; Justicia betonica;
pengikliman
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*Corresponding author; email: jam_sya@yahoo.com
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