Sains Malaysiana 44(5)(2015): 651–656

Molecular Characterization of Colletotrichum Isolates Associated with Anthracnose of Mango Fruit

(Pencirian Molekul Pencilan Colletotrichum Berkait dengan Antraknos Buah Mangga)

LATIFFAH ZAKARIA*, NURUL ZAADAH JUHARI, SUZIANTI ISKANDAR VIJAYA & INTAN SAKINAH MOHD ANUAR

School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia

Diserahkan: 16 Februari 2014/Diterima: 26 Februari 2015

ABSTRACT

Colletotrichum species are well-known causal agent of anthracnose. A study was conducted to determine the identity of Colletotrichum associated with anthracnose of mango (Mangifera indica) fruits. Thirty five Colletotrichum isolates were isolated from anthracnose lesion of two mango cultivars, Chokanan and Harum Manis. Based on the conidial morphology, two morphotypes (I and II) of C. gleosporioides were identified. Based on BLAST search of ITS regions and β-tubulin sequences, majority of the isolates showed 99-100% similarity with Colletotrichum sp. from mango and other hosts and three isolates, 100% similarity with C. asianum. From phylogenetic analysis using maximum likelihood method of combined datasets, the isolates from mango formed three clades, which corresponded to C. gloeosporioides sensu lato and C. asianum. Therefore, the present study showed that the isolates associated with anthracnose of mango belong to C. gleosporioides sensu lato and C. asianum.

Keywords: Anthracnose; β-tubulin; Colletotrichum; ITS regions; mango

ABSTRAK

Spesies Colletotrichum adalah agen penyebab utama antraknos. Suatu kajian telah dijalankan untuk menentukan identiti spesies Colletotrichum yang berkait dengan antraknos buah mangga (Mangifera indica). Tiga puluh lima pencilan Colletotrichum telah dipencilkan daripada lesi antraknos dua kultivar mangga, Chokanan dan Harum Manis. Berdasarkan morfologi konidium, dua morfotip (I dan II) C. gleosporioides telah dikenal pasti. Berdasarkan carian BLAST kawasan ITS dan gen β-tubulin, majoriti pencilan menunjukkan 99-100% kesamaan dengan Colletotrichum sp. daripada mangga dan perumah yang lain dan tiga pencilan, 100% kesamaan dengan C. asianum. Analisis filogenetik menggunakan kaedah kebolehjadian maksimum gabungan data, menunjukkan kesemua pencilan mangga membentuk tiga klad, yang sejajar dengan C. gloeosporioides sensu lato dan C. asianum. Oleh itu, kajian ini menunjukkan bahawa pencilan yang berkait dengan antraknos mangga adalah C. gloeosporioides sensu lato dan C. asianum.

Kata kunci: Antraknos; β-tubulin; Colletotrichum; kawasan ITS; manga

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*Pengarang untuk surat-menyurat; email: Lfah@usm.my