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Sains Malaysiana 46(9)(2017): 1457–1464 http://dx.doi.org/10.17576/jsm-2017-4609-14
Bioassay-Guided Fractionation of Emilia sonchifolia Extract
on the Induction of Ovarian Maturation in Fenneropenaeus merguiensis (Pemeringkatan Bioasai
Berpandu Ekstrak Emilia sonchifolia pada Aruhan Kematangan
Ovari dalam Fenneropenaeus merguiensis) LAMAI
MAIKAEO1,
WILAWAN
MAHABUSARAKAM1
& WILAIWAN CHOTIGEAT2* 1Department
of Molecular Biology and Bioinformatics, Faculty of Science, Prince
of Songkla University, Hat Yai, Songkhla, 90112, Thailand 2Department
of Chemistry, Faculty of Science, Prince of Songkla University Hat
Yai, Songkhla, 90112,Thailand Diserahkan:
31 Ogos 2016/Diterima: 17 Januari 2017 ABSTRACT This study was carried
out to identify the active compounds of Emilia
sonchifolia on induction of ovarian maturation in Fenneropenaeus
merguiensis. The crude extracts from dichloromethane and acetone
were investigated in vitro. The crude extract from acetone
induced up-regulation of shrimp ovarian peritrophin (SOP)
and translationally controlled tumor protein (TCTP)
genes, which were highly expressed during early phase of ovarian
development higher than dichloromethane extract. Furthermore, fraction
14 (F14) from acetone extract up-regulated both of the SOP and the TCTP genes
to the greatest extent. Leading to in vivo study, the effect
of ribosomal protein L10a (RPL10a), which acts as shrimp ovarian
stimulator plus with F14 on shrimp ovarian maturation was investigated
by injection. The best result was observed in the group that received
RPL10a plus with F14 at 0.8 μg/g body weight of shrimp. The
RPL10a plus F14 enhanced the shrimp ovaries from undeveloped stage
to 57% of early developing stage within 15 days. Meanwhile, the
control group remained 100% of the undeveloped stage. Hence, F14
seems to play a positive role in the induction of shrimp ovarian
maturation. The component of F14 was identified using mass spectroscopy
and presented as 1,2-benzenedicarboxylic acid; 2,4-di-tert-butyl
phenol; 2,4,5-trimethoybenzylidene;palmitic acid; 1-heneicosyl formate;
1-heptadecanol; ethyl-4-ethoxybenzoate and stearic acid. Keywords: E. sonchifolia; ovarian maturation; shrimp; SOP;
TCTP
ABSTRAK Penyelidikan ini telah dijalankan untuk mengenal pasti sebatian aktif Emilia sonchifolia pada induksi kematangan ovari dalam Fenneropenaeus merguiensis. Ekstrak mentah daripada diklorometana dan aseton dikaji secara in vitro. Ekstrak mentah daripada aseton meningkatkan aruhan peritofin ovari udang (SOP) dan secara translasi mengawal gen tumor protein (TCTP) yang dinyatakan pada fasa awal perkembangan ovari adalah lebih tinggi daripada ekstrak diklorometana. Selain itu, pecahan 14 (F14) daripada ekstrak aseton meningkatkan kedua-dua gen SOP dan TCTP ke tahap terbaik. Ini membawa kepada kajian secara in vivo dengan kesan ke atas protein ribosomal L10a (RPL10a), yang bertindak sebagai perangsang ovari udang tambahan F14 pada kematangan ovari udang yang dikaji melalui suntikan. Hasil terbaik yang diperhatikan dalam kumpulan yang menerima RPL10a dicampur dengan F14 pada 0.8 μg/g badan berat udang. RPL10a dan F14 meningkatkan ovari udang daripada peringkat mundur ke 57% peringkat awal pembangunan dalam masa 15 hari. Sementara itu, kumpulan kawalan kekal 100% pada peringkat mundur. Oleh yang demikian, F14 seperti memainkan peranan yang positif dalam aruhan kematangan ovari udang. Komponen F14 dikenal pasti menggunakan spektroskopi jisim sebagai asid 1,2-benzenadikarboksilik; 2,4-di-tert-butil fenol; 2,4,5-trimetoibenziliden; asid palmitik; format 1-heneicosil; 1-heptadekanol; etil-4-etoksibenzoat dan asid stearik.
Kata kunci:
E.
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