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Malaysian Rice Project
Gene/genome manipulation for yield
enhancement in rice
Study #2:
Identification
of yield related genes in cereals for yield enhancement in rice
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Objectives
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Identification of yield related genes in cereals/grass
- Gene transfer
- Evaluation of
traits in cultivars produced
Progress
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Isolation of individual genes:- The barley oxalate oxidase coding
sequence (Barley oxalate oxidase gene, HvOxOa) has been cloned.
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Cloning of large BAC insert:- The 107kb barley genomic fragment in
BAC450N4 has been restriction digested but we are unable to produce a
single fragment with any enzyme tested. Sub-fragments of the barley
genomic DNA have been cloned into the BIBAC plant transformation vector.
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Construction of binary constructs:- The barley oxalate coding
sequence (Barley oxalate oxidase gene, HvOxOa) has been cloned from
pGA1611 into a pCAMBIA plant expression vector, so that the new
construct (pUbiHvHoxo) is driven by a cereal ubiquitin promoter.
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Transformation of Agrobacterium:- The above binary vector construct
has been transformed into Agrobacterium and used to transform callus
induced from immature seed of rice variety MR81. Putative transformed
MR81 callus is being maintained towards the regeneration of rice
plantlets. Early putative transformants have failed to survive on
selective medium and /or are PCR negative. However further transformants
and regenerants are being generated and maintained.
F
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